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پاورپوینت در مورد animal culture

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پاورپوینت در مورد animal culture

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تعداد اسلاید : 66 اسلاید

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Animal Cell and Tissue Culture

In the name of God

Introduction

Cell culture is the process by which prokaryotic, eukaryotic or plant cells are grown under controlled conditions. But in practice it refers to the culturing of cells derived from animal cells.

Cell culture was first successfully undertaken by Ross Harrison in 1907

Roux in 1885 for the first time maintained embryonic chick cells in a cell culture

Historical events in the development of cell culture

1878: Claude Bernard proposed that physiological systems of an organism can be maintained in a

living system after the death of an organism.

1885: Roux maintained embryonic chick cells in a saline culture.

1897: Loeb demonstrated the survival of cells isolated from blood and connective tissue in serum

and plasma.

1903: Jolly observed cell division of salamander leucocytes in vitro.

1907: Harrison cultivated frog nerve cells in a lymph clot held by the 'hanging drop' method and

observed the growth of nerve fibers in vitro for several weeks. He was considered by some as

the father of cell culture.

1910: Burrows succeeded in long term cultivation of chicken embryo cell in plasma clots. He made detailed observation of mitosis.

Contd..

1911: Lewis and Lewis made the first liquid media consisted of sea water, serum, embryo extract, salts and peptones. They observed limited monolayer growth.

1913: Carrel introduced strict aseptic techniques so that cells could be cultured for long periods.

1916: Rous and Jones introduced proteolytic enzyme trypsin for the subculture of adherent cells.

1923: Carrel and Baker developed 'Carrel' or T-flask as the first specifically designed cell culture vessel. They employed microscopic evaluation of cells in culture.

1927: Carrel and Rivera produced the first viral vaccine - Vaccinia.

1933: Gey developed the roller tube technique

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